National Ecological Observatory Network (NEON) is a large facility to provide physical infrastructure and information infrastructure to science community to enable understanding and forecasting of climate change, land use change, and invasive species on continental-scale ecology. Stable isotopic measurement as one of the importance ecological research tools has been included in NEON design. An example of the biogeochemical cycle will be given in the presentation to show the stable isotopic measurements at NEON cross atmospheric, terrestrial and aquatic systems. Some technical details such as instrumentation, sampling frequency, system design will be briefly discussed. Some updates about NEON construction status, data availability, and time frame will also be provided.